Publications (375)
ARTICLE
Hemoglobins S and C interfere with actin remodeling in Plasmodium falciparum-infected erythrocytes
Marek Cyrklaff, Cecilia P. Sanchez, Nicole Kilian, Cyrille Bisseye, Jacques Simporé, Friedrich Frischknecht, Michael Lanzer
The hemoglobins S and C protect carriers from severe Plasmodium falciparum malaria. Here, we found that these hemoglobinopathies affected the trafficking system that directs parasite-encoded proteins to the surface of infected erythrocytes. Cryoelectron tomography revealed that the parasite generated a host-derived actin cytoskeleton within th(...)
hemoglobin S, hemoglobin C, Plasmodium falciparum, actin remodeling, infected erythrocytes
ARTICLE
[Prevalence and risk factors associated with infection by human immunodeficiency virus, hepatitis B virus, syphilis and bacillary pulmonary tuberculosis in prisons in Burkina Faso]
E A Diendéré, H Tiéno, R Bognounou, D D Ouédraogo, J Simporé, R Ouédraogo-Traoré, J Drabo
This purpose of this study was to determine prevalences and risk factors associated with infection by human immunodeficiency virus, hepatitis B Virus, syphilis and bacillary pulmonary tuberculosis in prisons in Burkina Faso. From February 20 to March 20, 2009, 300 prisoners over 18 years of age held in Ouagadougou were selected to take part in(...)
HIV; HBV; syphilis; tuberculosis; prison; prevalence; Burkina Faso; risk factors
ARTICLE
[Epidemiology, characterization of genotypes of human papillomavirus in a population of women in Ouagadougou]
C M R Ouedraogo, F W Djigma, C Bisseye, T Sagna, M Zeba, D Ouermi, S D Karou, V Pietra, F Buelli, N W Ghilat-Avoid-Belem, K Sanogo, J Sempore, R Moret, S Pignatelli, J-B Nikiema, J Simpore
Objectives: This work was carried out in order to determine the prevalence of different HPV genotypes in a population of women attending gynecological consultation.
Material and methods: From May to June 2010, cervical samples were obtained from 300 women attending gynecological consultation in two health centers in Ouagadougou. The strains(...)
Human papillomavirus; HPV genotypes; Epidemiology; Women; Ouagadougou; Gynecological consultation; PCR; Cervical infection
ARTICLE
Antimicrobial, antiplasmodial, haemolytic and antioxidant activities of crude extracts from three selected Togolese medicinal plants
Simplice D. Karou, Tchadjobo Tchacondo, Lassina Ouattara, Kokou Anani, Aly Savadogo, Amegnona Agbonon, Mossaclok Ben Attaia, Comlan de Souza, Mohsen Sakly, Jacques Simpore
Abstract
Objective: To investigate the antioxidant, antimicrobial, antiplasmodial, acute toxicity and haemolytic activities of methanolic extracts of three plants. Phytochemical analysis to determine the phenolic contents was also carried out.
Methods: The 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free radical scavenging, NCCLS broth microdilu(...)
Antimicrobial, antiplasmodial, haemolytic, antioxidant, methanolic extracts, Togolese medicinal plants, phytochemical analysis
ARTICLE
Seroprevalence of human immunodeficiency virus, hepatitis B and C viruses and syphilis among blood donors in Koudougou (Burkina Faso) in 2009
Nagalo MB, Sanou M, Bisseye C, Kaboré MI, Nebie YK, Kienou K, Kiba A, Dahourou H, Ouattara S, Zongo JD, Simpore J.
Background The high prevalence of numerous transfusion-transmitted infectious diseases such as human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV) and syphilis in sub-Saharan Africa affects the safety of blood for recipients. This study was undertaken with the aim of determining the seroprevalence of HIV, HCV,(...)
transfusion, blood donors, HBV, HCV, Burkina Faso
ARTICLE
Enteric parasites prevalence at Saint Camille Medical Centre in Ouagadougou, Burkina Faso
Simplice Damintoti Karou, Drissa Sanou, Djeneba Ouermi, Salvatore Pignatelli, Virginio Pietra, Remy Moret, Jean Baptiste Nikiema, Jacques Simpore
Objective: To assess the prevalence of parasitic infections among patients attending Saint Camille Medical Centre and to estimate co-parasitic infections rates.
Methods: From January to December 2009, stool samples were collected from 11 728 persons, aged from five months to 72 years and suffering from gastroenteritis. After macroscopic des(...)
Parasitic infections, intestinal parasites, co-infection, Giardia intestinalis, Burkina Faso, hygiene education
ARTICLE
[Molecular diagnosis of acquired human immunodeficiency virus (HIV) in pooled plasma from blood donors at the Regional Blood Transfusion Center in Ouagadougou, Burkina Faso]
B M Nagalo, C Bisseye, M Sanou, Y K Nebié, A Kiba, K Kienou, J D Zongo, J Simporé
Study objectives: The aim of this pilot study was to investigate the use of viral genome diagnosis of HIV-1 infection in blood donors in the regional blood transfusion center in Ouagadougou, Burkina Faso.
Methodology: This prospective study was carried out from August to December 2009 at the regional blood transfusion center in Ouagadougou(...)
HIV-1, RT-PCR, blood donors, Burkina Faso, molecular diagnosis, transfusion safety
ARTICLE
Nutritional and Hematological Recovery of Severely Malnourished Children with New Flour Formulas Developed at Ouagadougou (Burkina Faso)
W.R. Compaoré, P.A. Nikièma, A. Savadogo, H.I.N. Bassole, J. Mouecoucou, S. Pignatelli, J. Simporé, S.A. Traoré
A longitudinal study was carried out, which tested the efficacy of severe malnutrition recovery with Misola and two experimental flours produced with local products (seeds of Moringa oleifera and Cucurbita maxima, pulps of Adansonia digitata and Parkia biglobosa), supplemented with Zea mays (F1) or Pennisetum glaucum (F2). A total of 187 sever(...)
Formulated flours, growth and development, infants
ARTICLE
Validation, transfer and measurement uncertainty estimation of an HPLC–UV method for the quantification of artemisinin in hydro alcoholic extracts of Artemisia annua L.
Hermine Zime Diawara, Fernand Gbaguidia, Brigitte Evrard, Joëlle Quetin Leclercq, Mansourou Moudachirou, Benjamin Debrus, Philippe Hubertd, Eric Rozet
Malaria is the world’s most important parasitic infection with 500 millions cases annually and almost 2
millions death per year. This disease is more present in Sub-Saharan Africa where 90% of the infections
are found. Artemisinin and its semi synthetic derivatives (artemether, artesunate) have actually the most
powerful activity on malaria(...)
Method transfer, Measurement uncertainty, Method validation, Artemisia annua, Hydro alcoholic extracts
ARTICLE
Knowledge, attitudes and practice (KAP) regarding newborn feeding modalities in HIV-infected and HIV-uninfected pregnant women in sub-Saharan Africa: a multicentre study
L. R. Tomasoni, M. Galli, S. Declich, V. Pietra, F. Croce, S. Pignatelli, M. Fabiani, J. Simporé, M. Mabilia, E. O. Ayella, C. Caracciolo, G. Russo, G. Guaraldi, M. N. Gambirasio, V. Vullo, F. Castelli
Exclusive formula feeding, exclusive breastfeeding (EBF) with early weaning or the administration of antiretroviral therapy to lactating mothers and/or to breastfed newborns may lower postnatal HIV transmission. The aim of this study was to assess mothers' knowledge, attitudes and practice (KAP) on lactation in various real-life settings in su(...)
KAP, allaitement, VIH, femmes enceintes, Afrique sub-saharienne, EBF, prévention transmission postnatale
ARTICLE
Characterization of HIV-1 genotypes and antiretroviral drug-resistance mutations among patients in Burkina Faso
T S Kagone, H Hien, N Meda, P S Diagbouga, A Sawadogo, J Drabo, M Peeters, L Vergne, E Delaporte, C De Souza, M Gbeassor, J Simpore
The purposes of this study were: (1) to describe the genetic variability of HIV strains found in Burkina Faso, (2) to characterize non-B HIV strains mutation profiles selected by ARVs and (3) to detect possible resistances induced by ARV drugs. From 30 October 2002 to 20 November 2003, 132 HIV 1-positive patients taking Highly Active Antiretro(...)
HIV-1, genotypes, antiretroviral resistance, Burkina Faso, CRF02_AG, CRF06_cpx, HAART, polymorphism
ARTICLE
An ethnobotanical study of antimalarial plants in Togo Maritime Region
K Koudouvo, D S Karou, K Kokou, K Essien, K Aklikokou, I A Glitho, J Simpore, R Sanogo, C De Souza, M Gbeassor
Background: Malaria continues to be of great concern in Togo, as it accounts for high mortality rates, principally in children. Although plant-based medicines are the only means in major malarious regions of the country, still very little is known about the medicinal plants and medical practices of the indigenous people.
Objective: The pres(...)
Ethnobotany, antimalarial plants, traditional medicine, Togo, malaria, decoction, fidelity level, indigenous knowledge
ARTICLE
In vitro antiplasmodial activity of crude extracts from Togolese medicinal plants
Koffi Koudouvo, Simplice D Karou, Denise P Ilboudo, Kouami Kokou, Kodjo Essien, Kodjo Aklikokou, Comlan de Souza, Jacques Simpore, Mensavi Gbéassor
Objective: To investigate the antimalarial effect of a few plants in Togo folk medicine.
Methods: After ethnobotanical survey, Opilia celtidifolia, Pavetta corymbosa (P. corymbosa) and Tamarindus indica (T. indica) were selected for screening. In vitro antimalarial tests were performed on crude extracts against fresh clinical isolates of Pl(...)
Antiplasmodial, Plasmodium falciparum, plantes médicinales togolaises, activité in vitro, alkaloïdes
ARTICLE
Développement et validation d’une méthode quantitative de dosage par Chromatographie Liquide à Haute Performance Ultra-violet (CLHP-UV) de l’artémisinine dans Artemisia annua cultivé au Bénin
Hippolyte GANSE, Fernand GBAGUIDI, Taofiki AMINOU, Hermine ZIME, Mansourou MOUDACHIROU, Joëlle QUETIN-LECLERCQ
Artemisia annua, espèce de la famille des asteraceae est une plante dont les feuilles contiennent une
lactone sesquiterpénique appelée artémisinine qui est efficace contre toutes les souches de parasites
responsables du paludisme. Cette étude nous a permis de développer et de valider une méthode de
Chromatographie : la méthode de Chromatogr(...)
CLHP-UV, artémisinine, artemisia annua
ARTICLE
In vitro anti Mycobacterium tuberculosis H37Rv activity of Lannea acida A. Rich from Burkina Faso
L Ouattara, J Koudou, D S Karou, L Giacò, G Capelli, J Simpore, M Fraziano, V Colizzi, A S Traore
The cytotoxic and anti-Mycobacterium tuberculosis H37Rv activities of hydro-alcoholic extract of Lannea acida A. Rich (Anacardiaceae) were assessed. The cytoxicity evaluation was carried out on THP1 monocytoid cell line (after 24 h at 1; 5 and 10 microg mL(-1)) and showed only a slight modification of lactate dehydrogenase (LDH) release. The r(...)
Mycobacterium tuberculosis, Lannea acida, activité antimycobactérienne, cytotoxicité, extrait hydroalcoolique