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Pseudomonas aeruginosa is a bacterium present in several ecological niches causing opportunistic infections in humans. In developing countries like Chad, this bacteria is responsible for various human pathologies. High-priority pathogens, such as Salmonella and Shigella, represent a particularly heavy burden in low- and middle-income countries, as do Pseudomonas aeruginosa and Staphylococcus aureus, which pose serious problems in health centers. The objective of this study was to evaluate the multi-antibiotic resistance index and to carry out a molecular characterization of Pseudomonas aeruginosa isolated in pathological products from Chad using by rep-PCR (GTG)5. Antibiotic resistance phenotypes were obtained by interpreting the results of our previous study on antimicrobial resistance of Pseudomonas aeruginosa isolated from human infections in N'Djamena, Chad. In this study, two phenotypes were obtained that is XDR and MDR. Thus, 24/36 of strains came from urine and 29/36 strains were MDR. The multi-antibiotic resistance (MAR) indexes varied from 0.33 to 0.86. The rep-PCR allowed good discrimination of Pseudomonas aeruginosa strains in this study. These isolates were grouped based on characteristic bands appearing in their genetic fingerprint profiles. In this study, Pseudomonas aeruginosa strains isolated from urinary tract infections and pus were classified as XDR and MDR type strains.

In Burkina Faso, chemical inputs are used massively in market gardening to increase the yields. The misuse of these chemicals is responsible of contamination of market garden products, leading to the rapid rotting of vegetables. The objective of this study was to determine the pesticides in chemical inputs applied on vegetables produced in Ouagadougou City. To do this, lettuce has been sampled from producers in three sites (Tanghin, Boulmiougou and Kossodo) and pesticides amounts have been analyzed using Gas Chromatography coupled with mass spectrometry. Chemical analyses have carried out on three lettuce samples and the extraction of chemical input residues was done according to QuEChERS (Quick Easy Cheap Effective Rugged and Safe) method. Results revealed the presence of six (06) pesticide molecules (chlordimeform, quintozene, lindane, cypermethrin, α-cypermethrin, δ-deltamethrin) in Boulmiougou sample, four (04) molecules (methomyl, cypermethrin, α-cypermethrin, δ-deltamethrin) in Kossodo sample and three (03) molecules (cypermethrin, α-cypermethrin, δ-deltamethrin) in Tanghin sample. In this regard, analysis of pesticide amount revealed that the quantity of molecules was acceptable for the consumer, but these pesticides could contribute to the rapid rotting of our vegetables which generates economic and social losses for the country.

Introduction: Klebsiella pneumoniae is an encapsulated Gram-negative bacterium that is responsible for numerous infections in healthcare facilities worldwide and is frequently isolated. The World Health Organization has listed K. pneumoniaeas as a critical antibiotic resistant bacterial pathogen for which new antibiotics are urgently needed. This study aimed to use molecular tools to identify and examine antibiotic resistance in clinical strains of K. pneumoniae. Methodology: A total of 15 unduplicated K. pneumoniae strains isolated from patient samples with multidrug-resistant (MDR) profiles were subjected to polymerase chain reaction (PCR) to amplify the most common carbapenem resistance genes. (GTG)5 PCR and phylogenetic analysis were performed to identify the genetic relationship between the strains. Results: All strains yielded a (GTG)5-PCR profile, and this allowed us to group these strains into 8 groups according to the size and number of characteristic bands. Phylogenetic analysis was done using the free software UPGMA and a single bacterial clone with a correlation coefficient of over 97% was identified. New Delhi metallo-beta-lactamase NDM-like (blaNDM) carbapenem resistance genes were detected in three strains of K. pneumoniae, which represented a resistance rate of 20%. However, carbapenemases type A [Klebsiella pneumoniae carbapenemase (KPC) and imipenem-hydrolysing beta-lactamase (IMI), type D [oxacillinase-48 (OXA-48)], and other metallo-β-lactamase [Verona integron-encoded metallo-beta-lactamase (VIM), and enzyme active on imipenem (IMP)] were not detected. Conclusions: We identified and grouped the blaNDM resistance genes of Klebsiella pneumonia strains.

The analytical expression for the degree of multivariate discordance in probability has a high level of mathematical elegance. This is why we were interested in the degree of discrepancy. In addition, while working on this expression, an application to the Bernstein copula appeared more accessible. We therefore modeled the expression for the Bernstein copula and the degree of discordance.

The present work analyzes the energy production of three 50kWp photovoltaic (PV) power plants which are placed in three climate zones. The Sudano-Sahelian and Sudanian climates have lower average temperatures than the Sahelian environment. The Sudanian climate, next the Sudano-Sahelian zone, and finally the Sahelian region, have the best performance for PV power plants. The performance of silicon PV cells may suffer due to temperature. The Sahelian climatic zone experiences its highest levels of PV output between April and October due to longer sunshine hours and a reduction in sand dust. In the Sudano-Sahelian region, PV output is at its peak between May and October. The best PV generation in the Sudanian environment occurs between June and November. The theoretical forecasting for the various climate zones is consistent with the PV mini-grid real energy output in various situations. The humidity, cloud cover, and rainfall in all climatic zones will make August the worst month for PV energy generation. Due to the type of load compared to the Sudanian climate where the PV power plant is situated in a university, the usage of batteries is crucial in the Sudano-Sahelian climate.

Greenhouse gas (GHG) emissions from prescribed fires are poorly investigated, resulting in a high uncertainty in GHG budgets. Using, a carbon mass balance approach and experimental prescribed fires in 80 plots, this study assessed carbon emissions and established emission factors (EFs) for carbon dioxides (CO2), carbon monoxide (CO), and methane (CH4) across climate zones and vegetation types. In grass and shrub savannas, fires could burn intensely due to the lower moisture content and continuous spatial distribution of biomass fuel, causing greater carbon emissions with 1.61 ± 0.13 t C ha−1 and 1.01 ± 0.13 t C ha−1, respectively. Despite their low carbon emissions, tree savannas (1658.17 ± 11.13 g kg−1) and woodlands (1629.94 ± 12.23 g kg−1) have the highest EFs, which can be attribute to the high carbon content of biomass fuel in these vegetation types. Vegetation types and their interaction with climate zones have a substantial impact on carbon emissions and carbon species EFs, and should therefore be considered in assessing GHG emissions from fires. The findings from this study provide a useful basis for improving the national measurement, reporting, and verification of GHG emissions and for improving the measurement of the global balance of GHG emissions from fires.

La littérature sur l’impact de l’assurance-vie sur la performance économique dans les pays en développement reste peu concluante. Dans cet article, nous examinons la possibilité que les pays suivent des régimes de croissance différents, et testons l’hypothèse selon laquelle l’assurance-vie affecte différemment la croissance économique dépend du régime de croissance auquel appartient une économie. Ainsi, en utilisant un modèle de mélange fini sur 34 pays d’Afrique subsaharienne au cours de la période 1999-2022, nous identifions deux régimes de croissance. L’assurance-vie a un effet positif et significatif dans le premier, tandis que dans le second, elle n’a pas d’effet significatif. De plus, nous utilisons le modèle à effet aléatoire de Mundlak (1978) pour capter le fait que le développement financier augmente la possibilité qu’un pays appartienne à un régime dans lequel l’assurance-vie influence positivement la croissance économique. Ces résultats suggèrent que le secteur financier peut être un canal permettant d’accentuer l’effet de l’assurance-vie sur la croissance.

High solubility in water and physiological fluids is an indispensable requirement for the pharmacological efficacy of an active pharmaceutical ingredient. Indeed, it is well established that pharmaceutical substances exhibiting limited solubility in water are inclined towards diminished and inconsistent absorption following oral administration, consequently resulting in variability in therapeutic outcomes. The current advancements in combinatorial chemistry and pharmaceutical design have facilitated the creation of drug candidates characterized by increased lipophilicity, elevated molecular size, and reduced aqueous solubility. Undoubtedly, the issue of poorly water-soluble medications has been progressively escalating over recent years. Indeed, 40% of the top 200 oral medications marketed in the United States, 33% of drugs listed in the US pharmacopoeia, 75% of compounds under development and 90% of new chemical entities are insufficiently water-soluble compounds. In order to address this obstacle, formulation scientists employ a variety of approaches, encompassing both physical and chemical methods such as prodrug synthesis, salt formation, solid dispersions formation, hydrotropic substances utilization, solubilizing agents incorporation, cosolvent addition, polymorphism exploration, cocrystal creation, cyclodextrins complexation, lipid formulations, particle size reduction and nanoformulation techniques. Despite the utilization of these diverse approaches, the primary reason for the failure in new drug development persists as the poor aqueous solubility of pharmaceutical compounds. This paper, therefore, delves into the foundational principles that underpin the implementation of various formulation strategies, along with a discussion on the respective advantages and drawbacks associated with each approach. Additionally, a discourse is provided regarding methodological frameworks for making informed decisions on selecting an appropriate formulation strategy to effectively tackle the key challenges posed during the development of a poorly water-soluble drug candidate.

In Burkina Faso, products and nests of insect are used for therapeutic purposes in traditional medicine. However, this use by local populations is marginal and empirical. The objective of this study was to evaluate the antimicrobial activity of extracts of bees’ products and termite nests. Thus, the collected termite nests were finely ground. Hydroethanolic extraction of bioactive molecules with potential antibacterial activity was performed according to standard methods. The agar diffusion method was used to test the antibacterial activity of hydroethanolic extracts of honey bee, bee wax, propolis, and termite nests against 22 pathogenic strains by inhibition diameter. Imipenem and nystatin were used as a positive control for bacterial and fungal strains respectively. The extraction yields varied from 7.33 to 35.39% depending on the content of soluble matter. All extracts of bees’ products and termite nests tested showed inhibitory activities. The inhibition diameters varied depending on the extract and strain tested. The largest diameter of inhibition (26 ± 0.0 mm) was obtained using the nest extract of Macrotermes bellicosus against Salmonella Typhimurium ATCC14028. The lowest diameter of inhibition was 7 ± 0.0 mm obtained with honey extract against Pseudomonas aeruginosa ATCC27853. Index multi-resistance of the extracts tested were between 0.2 and 0.6. Interestingly, the inhibition diameters and activity coefficient of certain products and nest extracts of insects were sometimes greater than those of imipenem/nystatin against the strains tested. This study revealed the antimicrobial potential of termite nest extracts and hive products against pathogens.

The construction of multivariate distributions with arbitrary margins has been a problem of interest to statisticians for many years, but nowadays, by virtue of Sklar’s theorem, this problem can be reduced to the construction of a copula. However, there is no general method for constructing a copula. In order to provide a partial solution to this problem, we present in this article, a construction of a new class of ratio-type copulas called a class of copulas with horizontal or vertical section of a homographic function. Indeed, the horizontal section of the copulas of this class can be considered as a homographic function of variable u whose coefficients are functions of v (formula (8)) and the vertical section can be considered as a homographic function of variable v whose coefficients are functions of u (formula (9)). This class can be also considered as a generalization of Ali-Mikhail-Haq family of copulas. We present some examples of copulas in this class.