Present study aim to carry out an ethnobotanical survey into medicinal plant recipes used in the treatment of hemorroidal pathologies and select the most cite for phytochemical and biological investigation, using ethanolic and
hydroethanolic extracts. Phytochemistry study concerned tube test and polyphenolic quantification. Biological one concerned the antioxidant and antibacterial activity. The Folin-Ciocalteu reagent, AlCl3 and vanillic acid were used respectively for the detremination of total phenolics, flavonoids and tanins content. Anti-DPPH*and anti-FRAP were used to evaluate the antioxidant activity and liquid dilution method for the antibacterial activity.55 medicinal plant recipes were identified with T. emetica and C. sieberiana the most cited species. Saponosides, flavonoids, tannins and alkaloids were identified in the both extracts. Among the total extracts, C. sieberiana gave the high content of phenolic compounds (75.76 ± 0.44 mgEAG/100mg) and flavonoids (0.63 ± 0.36 mgEQ/100mg) respectively with the ethanolic and hydroethanolic extracts on the other hand the best tannin content was observed by the recipe hydroethanolic extract (47.48 ± 3.81 mgEC/100mg). Regarding the antioxidant activity the best anti-DPPH* inhibition percentage was obtained with the hydroethanolic extract of C. sieberiana (88,99 %) and the best iron reducing power with the recipe of ethanolic extract (6,52 mmolAA/g). The best Minimum Inhibitory Concentration of 0,78mg/mL was observed on the species of E. coli and K. pneumoniae with the ethanolic extract of C. sieberiana. Also the best Minimum Bactericidal Concentration (25 mg/mL), was observed on the majority of bacterial strains and with all of our extracts. Present findings could partially justify the traditional uses of these plants in the treatment of hemorroidal pathologies.

Phytochemistry, Antibacterial, medicinal plant recipes, Antihemorrhoidal