Te emergence and spread of carbapenem resistance in Gram-negative bacilli such as Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii, and Pseudomonas aeruginosa through the production of carbapenemases is a global phenomenon. It threatens patient care and leads to therapeutic impasses. Tis study aims to genotypically determine the prevalence of the most frequent carbapenemase genes among multidrug-resistant E. coli strains isolated from patients at a biomedical analysis laboratory. A total of ffty-three unduplicated E. coli strains isolated from patient samples with a multidrug-resistant (MDR) profle were subjected to polymerase chain reaction (PCR) testing for carbapenem resistance genes. Tis study allowed us to identify ffteen strains carrying resistance genes among the ffty-three E. colistrains. All ffteen strains produced the metallo-β-lactamase enzymes; this represents a rate of 28.30% of study strains. Among these strains, ten carried the NDM resistance gene, NDM and VIM genes
were detected in three strains and VIM was identifed in two strains of E. coli. However, carbapenemases A (KPC and IMI), D (OXA-48), and IMP were not detected in the strains studied. Tus, NDM and VIM are the main carbapenemases detected in the strains in our study.