Bemisia tabaci Gennadius is a major plant pest in many agricultural systems worldwide. It is a complex of cryptic species that differ in many ecological respects, including damage-causing potential. Monitoring the genetic composition of B. tabaci populations in the field is, therefore, essential for achieving effective control. Sequencing is costly and time consuming; thus, efficient diagnostic tools must be developed to perform epidemiological studies involving hundreds of individuals. Here, we describe a polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) method for identifying all the putative species comprising the Africa/Middle East/Asia Minor group, including those that are the most devastating crop pests. Moreover, intra-specific diversity associated with specific symbiotic bacteria known to manipulate insect host phenotypes can also be detected. In …