Resistance to insecticide is the main concern for vectors control based on insecticides. We conducted a two-year monitoring on the resistance to insecticides of Anopheles gambiae and Anopheles funestus in five localities of Burkina Faso. The susceptibility to insecticides was performed using WHO test. The knock-down time (KDT) and after 24h mortality was assessed. Mosquitoes were PCR-tested for species, molecular forms and kdr gene identification. The specific composition of Anopheles gambiae varies according to locality and year of collection. The M form is more represented than the S form and An. arabiensis. There is a general increasing of the survival rate to DDT and the frequency of the kdr mutation in Anopheles gambiae. An. funestus was fully susceptible to DDT and pyrethroids in Koubri where it has been collected. These results highlighted the need for monitoring …