Prevalence, species diversity, and antimicrobial resistance profiles of Campylobacter spp. among children under five years of age with acute diarrhea in Ouagadougou, Burkina Faso
- AMERICAN SOCIETY FOR MICROBIOLOGY Journals , 14 (8) : 1-13
Résumé
Campylobacter species are major causes of pediatric gastroenteritis worldwide, yet their burden remains underestimated in West Africa due to diagnostic challenges. This study aimed to determine the prevalence, species diversity, and antimicrobial resistance (AMR) profiles of Campylobacter spp. among children under 5 years of age with acute diarrhea in Ouagadougou, Burkina Faso. From 1 May 2023 to 30 April 2024, 383 stool samples were collected at the Centre Hospitalier Universitaire Pédiatrique Charles de Gaulle. Samples were analyzed using standard culture on Karmali selective agar and biochemical identification via the API Campy system. AMR testing was performed using the disk diffusion method. Campylobacter spp. were detected in 33 samples (8.6% prevalence). Infection was significantly associated with the 12–23 months age group (48.5%; P < 0.05). Campylobacter jejuni was the most common species (42.4%), followed by C. coli (21.2%) and C. fetus subsp. fetus (21.2%). Other species included C. hyointestinalis (6.1%), C. sputorum (6.1%), and C. lari (3.0%). Alarmingly, 100% of isolates were resistant to ampicillin, erythromycin, and tetracycline. High resistance rates were also observed for amoxicillin-clavulanic acid (93.9%) and ciprofloxacin (75.8%). Gentamicin showed the highest susceptibility (57.6%). All isolates (100%) were classified as multidrug-resistant (MDR). These results highlight a high prevalence of MDR Campylobacter in Ouagadougou, with total resistance to erythromycin, the first-line treatment. The identification of C. fetus at 42°C suggests a high environmental and zoonotic pressure. These findings underscore the urgent need for enhanced genomic surveillance and updated clinical management guidelines for pediatric diarrhea in the region.
Mots-clés
Campylobacter, diarrhea, children, antimicrobial resistance, multidrug resistance, One Health, Burkina Faso