Evaluation of the Effects of Growth Media on Potato (Solanum tubérosum L.) Plantlets Production in Burkina Faso

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Résumé

Aims: To identify an appropriate culture medium suitable for micropropagation of plantlets with good
root proliferation and stem growth of potato.
Study Design: In Burkina Faso, potato growers face difficulties in obtaining potato seeds. In
general, most of the imported seeds partially meet the quality requirements of potato production.
Furthermore, these seeds are not accessible in terms of cost and are unavailable in time. In vitro
culture can remedy this problem by providing quality seeds.
Place and Duration of Study: This study was conducted in the laboratory of the Institut de
l’Environnement et de Recherches Agricoles (INERA) at Kamboinsé in Burkina Faso, between
August to September 2022.
Methodology: Explants from in vitro sprouts (1 cm) of the same generation were used as explants
were cultured different media. The media used were as follows: M0= 4.4 g l-1 MS medium + 7 g l-1
agar; M1= M0 + 30 g l-1 sucrose; M2= M0 + 0.5 mg l-1 3 indole butyric acid (IBA) and M3= M0+30 g
l-1 sugar + 0.5 mg l-1 IBA. The temperature of 22 ± 1oC, 70% relative humidity, a photoperiod of 16 h
and a lighting intensity of 1200 lux.
Results: this study indicated that M1 and M3 regenerated in vitro plantlets better those in M0 and
M2 did. Sucrose had a positive effect on root length, stem diameter, number of nodes and number
of opened leaves. These parameters strongly differentiated M1 from the other media. IBA had a
positive effect on the root proliferation of in vitro plantlets in the M2 medium. The combined effect of
sucrose and IBA had even greater effects on stem height, number of roots, number of leaves open
and weight of in vitro plantlets, which strongly differentiated medium M3 from the other media.
Conclusion: M3 proved to be the best medium for in vitro plantlet production in the 10 cultivars of
potato.

Mots-clés

Micropropagation; sugar; auxin IBA; In vitro culture; potato.