GTG)5-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso

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Résumé

bstract
Background: Escherichia coli (E. coli) is the most common bacterial species implicated in various types of infections including septicemia, gastroenteritis, urinary tract infections, meningitis and others pathologies. These involve
several bacterial clones with multidrug resistance making them difficult to treat. The aims of this study was to perform
molecular typing of E. coli strains using universal primer (GTG)5. In this study, 53 E. coli strains were collected from inpatients and outpatients. The test of antimicrobial sensibility was realized using CA-SFM /EUCAST method and strains
were identified by conventional microbiological tests. The carbapenemase-producing strains were demonstrated by
phenotypic method. Bacterial DNA was extracted by boiling method. (GTG)5-PCR was used for strain subtyping. The
DendroUPGMA software was used for grouping of strains from the genetic fingerprints obtained by (GTG)5-PCR.
Results: Antibiotic susceptibility test revealed that all strains were multi-drug resistant (MDR). Its strains showed
resistance to at least three different families of antibiotics. Of this MDR strains, only one was a metallo-β-lactamase
producer. The dendrogram obtained using genetic fingerprinting allowed the E. coli strains to be grouped into 22
clusters (G1 to G22).
Conclusion: The (GTG) 5-PCR assay enabled rapid molecular typing of E. coli strains. The strains of E. coli typed in this
study would belong to different clones.

Mots-clés

E. coli, Multidrug resistance, (GTG)5-PCR, Molecular typing