Determination of The Radical Scavenging Activities and Identification of Anthocyanins from Hexalobus monopetalus Ripe Fruits

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Résumé

A wild fruit from classified forest of Dindéresso was analyzed for total phenolics, flavonoids,
anthocyanins compounds using the Folin-Ciocalteu reagent, spectrophotometric method of
Zhishen and colleagues and by the differential pH method respectively. Free radicalscavenging activities of studied fruits extracts were estimated using diammonium 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) salt method. Three major anthocyanins were
identified using high performance liquid chromatography coupled with spray ionization
interface mass spectrometry. Three identified anthocyanins in fruit were reported to be
cyanidin 3-O-(p-coumaroyl) glucoside, pelargonidin 3-O-glucoside and pelargonidin 3-Orutinoside. In addition, H. monopetalus fruit contained of about 1165±3.1 mg of GAE per
100 g of fresh fruit, 4490±20.2 mg of QE per 100 g of fresh fruit, and 36±0.17 mg of cyanidin
3-O-glucoside equivalents per 100 g of fresh fruit. Total anthocyanin extract had an EC50 =
4.24 mg per mL and a TEC50 time of 21 minutes (intermediate reaction). This free radicalscavenging activity was very low compared to those of the references used (0.024 and 0.034
mg/mL respectively for ascorbic acid and Trolox). The low antiradical activity and reactivity
of the H. mucronata extract could be explained by several factors. In any case, fruits of this
species were potential sources of natural bioactive substances having beneficial effects on
the health of consumers.

Mots-clés

Hexalobus monopetalus ; ripe fruits ; HPLC-MS/MS ; anthocyanins ; antioxidants