Background and Objective: Solenostemon rotundifolius was a well-known medicinal plant with a multi-use and limited knowledge in phytochemicals. For this purpose, it was carried out a phytochemical study for highlight the secondary metabolites variation including phenolics, flavonoids and flavonols contents. Materials and Methods: The plant material consisted of 121 accessions of S. rotundifolius including 40 from Burkina Faso and 81 from Ghana. One gram of leaf powder from each accession was extracted in 10 mL methanol. The polyphenolic, flavonoids and flavonols contents and the DPPHC inhibition effects were studied using spectrophotometric methods. Analysis of Variance (ANOVA) was carried out and the differences were verified using the Student-Newman-Keuls test. Results: The polyphenolic contents ranged from 174.09±3.70 mg GAE/g for accession UE52 to 0.48±0.07 mg GAE/g for accession UE134. Interestingly, despite the new edaphic and climatic conditions, there was a significant variability in the phytoconstituents production of the accessions according to their climatic zones of origin. Thus, they could be classified according to this order: Sahelian>SudanoSahelian>Sudanian>Sudan-Savanna>Guinea Savanna. Cluster analysis based on the presence of phenolic compounds revealed the medicinal potential of S. rotundifolius leaves. Conclusion: The study of phytochemical screening revealed the various secondary metabolites including polyphenols, flavonoids and flavonols in the leaves of Solenostemon rotundifolius and justified partially its use
in traditional medicine.

Solenostemon rotundifolius, phytoconstituents, antiradical, Gampéla, polyphenolic content,