Carica papaya and Balanites aegyptiaca seeds extract have several uses, from food to
traditional medicine. This study aims to determine their phytochemicals compound and
appreciate their antimicrobial properties against clinical strains. Polyphenolic content was
determined with standard methods while DPPH radical scavenging activity is used to
appreciate the antioxidant properties, using ascorbic acid as standard. Antimicrobial activities
were performed according to the inhibition diameters, using agar diffusion method. Minimum inhibitory
concentrations and Minimum fungicidal concentrations were established based on broth dilution
methods. The extraction yield is ranged between 6.3 and 20.3%. The results shown polyphenolic
content varying from 1256.13±21.09to 2315.81±53.52µg GAE/100 mL for Carica papaya and
from 303.98±0.93 to 948.06±3.23 µg GAE/100 mL for Balanites aegyptiaca while the
antioxidant activity is ranged between 31.76±2.35 and 58.47±4.24 µg AEAC/100 mL for Carica
papaya and from 29.80±0.32 to 78.44±2.81 µg AEAC/100 mL for Balanites aegyptiaca.
Infusion’s extracts always have the highest values of polyphenols content and antioxidant
activity than decoction extracts, be it papaya or balanites seeds. Neitheir flavonoids content
nor antibacterial activity was found. The inhibition diameter varies from 14.5±0.5 to 40.5±0.5
mm for papaya seeds extracts and from 16.5±0.5 to 21.0±0.5 mm for balanites seeds extracts
without a significative effect of mixture for both extracts. MICs vary from 11.71±0.1 to 75.0±0.0
mg. mL-1 for Carica papaya seeds extracts with 11.72±0.2 to 37.30±0.2 mg. mL-1 and 18.74±0.1
to 100±0.0 mg.mL-1 for Balanites aegyptiaca. Mixture extracts presented intermediaire values.
Decoction extracts have lowest MICs values and infusion reveals itself to be the sweetable
aqueous extraction techniques but the appropriate infusion time need to be highlight

Carica papaya Balanites aegyptiaca Seeds extracts Phytochemical antifungal