AepraervtimouesnsttuodfyMheadsirceaploNrteadntohtecinhtneorelsotginyg, SreclhaxoaonltoeffAecdtvoafntchedhTyedcrohanlocolohgoliiecseixntrMacetdfriocmineW,aFltahseariUa ninidviecrasiLty. (oMfaMlvead-- icaelaeS)clieanfcyest,emFas,sa,pIlarnatnwith several therapeutic uses. The present study aimed to investigate the preventive effect of this
plant using an ex vivo model of the rat trachea. Two agonists, acetylcholine (10-6 - 1.5×10-5 M) and barium (10-5- 10-1 M) were Received: 30 May 2021 Revised:14 Jul 2021 Accepted: 16 Jul 2021
used to induce contractions. The preventive effect was assessed on rat tracheal rings pretreated with hydroalcoholic extract
(1.92 mg/mL), glibenclamide, atropine, and papaverine, all at 10 μM. Acetylcholine and barium provoke contractions in a
ly. The EC50 values were 3.711 ± 0.823 μM and 9.502 ± 12.354 mM, respectively, for acetylcholine- and barium-induced
Abstract
concentration-dependent manner, with a maximum contractile response of 3.953 ± 0.692 g and 2.999 ± 0.326 g, respective-
contraction. Glibenclamide caused a rightward shift of the acetylcholine-response curve, followed by a reduction of the
Cancer is the second leading cause of death worldwide, and due to the emergence of
maximum contraction (from 3.953 ± 0.692 g to 3. 116 ± 0.244 g). The hydroalcoholic extract, atropine (ATROP), papaver-
resistance to synthetic drugs in different cancers, developing new green drugs have be-
ine (PAP), and their combinations induced a complete suppression of the contractile response to acetylcholine (p

Waltheria indica Rat trachea Acetylcholine Barium chloride Glibenclamide Papaverine