Alternatives to Pyrethroid Resistance: Combinations of Cymbopogon nardus and Ocimum americanum Essential Oils Improve the Bioefficiency Control Against the Adults’ Populations of Aedes aegypti (Diptera: Culicidae),
Auteur(s): Mahamoudou Balboné, Dieudonné Diloma Soma, Samuel Fogné Drabo, Moussa Namountougou, Hamadou Konaté, Georges Benson Meda, Ignace Sawadogo, Rahim Romba, Etienne Bilgo, Roger C. H. Nebié, Imaël H. N. Bassolé, Roch K. Dabire, and Olivier Gnankine
Auteur(s) tagués: Imaël Henri Nestor BASSOLE ;
Résumé

Dengue vector control strategies are mostly based on chemicals use against Aedes aegypti populations. The current study aimed at investigating the insecticidal effects of essential oils (EOs) obtained from five plant species, Cymbopogon citrates (D. C.) Stapf. (Poaceae), Cymbopogon nardus (Linn.) Rendle (Poaceae), Eucalyptus camaldulensis Linn. (Myrtaceae), Lippia multiflora Moldenke (Verbenaceae), and Ocimum americanum Linn. Lamiaceae, and combinations of Cymbopogon nardus and Ocimum americanum on Ae. aegypti populations from Bobo-Dioulasso. For this purpose, adults of the susceptible and field strains of Ae. aegypti were tested
in WHO tubes with EO alone and binary combinations of O. americanum (OA) and C. nardus (CN; scored
from C1 to C9). The extraction of the essential oils was done by hydrodistillation, and their components were
determined by GC/MS. Among the 5 EOs tested, L. multiflora essential oil was the most efficient, with KDT
50 values below 60 min on all Ae. aegypti strains tested, and also with a rate of mortality up to 100 and 85% for
Bora Bora and Bobo-Dioulasso strains, respectively. This efficacy may be due to its major compounds which
are with major compounds as β-caryophyllene, p-cymene, thymol acetate, and 1.8 cineol. Interestingly, on all
strains, C8 combination showed a synergistic effect, while C2 showed an additive effect. These combinations
exhibit a rate of mortality varying from 80 to 100%. Their toxicity would be due to the major compounds and
the putative combined effects of some major and minor compounds. More importanly, L. multiflora EO and
combinations of C. nardus and O. americanum EO, may be used as alternatives against pyrethroid resistant
of Ae. aegypti.

Mots-clés

resistance essential oil combination major compound

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