The function of cytochrome P450 (CYP) enzymes, one of the major catalysts in drug metabolism, is
significantly influenced by genetic polymorphisms leading to substantial inter-individual variability in drug
response and/ or adverse reactions activity. The aim of this study was to determine the frequency of the CYP2B6
516GT allele in patients living with HIV receiving treatment at the Medical Center of Camp General Aboubacar
Sangoule Lamizana for whom the treatment was changed after the initiation of treatment because of the
appearance of side effects. 155 HIV-positive patients were enrolled. Genomic Deoxyribonucleic acid (DNA)
extraction from blood was performed using the GenJET® Genomic DNA according to the manufacturer’s
instructions. Genotyping for the cytochrome P450 variant alleles was performed using predesigned primers. The
amplification was carried out by Polymerase Chain Reaction (PCR), while the differentiation between the alleles
was carried out after digestion with restriction enzymes by electrophoresis. The results obtained showed. Among
these patients, 61 changed treatments during their follow-up. The majority of the 61 patients, 32 patients, received
an initial treatment regimen based on the AZT (3'-Azido-3'-Deoxythymidine)/3TC (Lamivudine)/
NVP(Nevirapine) combination, followed by 18 patients for the TDF (tenofovir)/3TC (lamivudine)/
EFV(Efavirenz) regimens. Currently, the majority of patients, 48 patients, have benefited from a therapeutic
regimen based on the TDF (tenofovir)/3TC (lamivudine) /DTG(Dolutegravir) combination, followed by 11
patients for the TDF (tenofovir)/3TC (lamivudine)/EFV(Efavirenz) regimens. The distribution of the major allele
CYP2B6*T was 68%, and the minor CYP2B6*G allele was 32%. Heterozygote (GT genotype) and Homozygous
mutants (TT genotype) and Heterozygote were 31% and 52,5%. Studies could be continued to set up cytochrome
mutation detection kits to anticipate the onset of side effects and adapt treatments

CYP2B6 516 G>T Correlation clinical outcomes HIV patients