The rapid evaluation of antioxidant activities of plant extracts is of interest to many researchers in the field of phytochemistry. Electrochemical methods appear to be an alternative to the classic 2,2-diphenyl-1picryl-hydrazyl (DPPH) method, which is considered complex and constraining to be easily implemented. Indeed, using cyclic voltammetry has already been proved to be relatively easy to implement to assess the antioxidant activity of plant extracts with no interference from solvents. For this study, the main objective is the use of Differential Pulse Voltammetry (DPV) to determine antioxidant activities as an alternative electrochemical method for evaluation of antioxidant activities of plant extracts. So, raw extracts of Mareya micrantha leaves, as well as their fractions were used. Under the optimal use conditions of both methods, antioxidant activity and particularly minimal concentrations of analyte inhibiting 50% of radicals (IC50) were evaluated. For crude extract then hexane, dichloromethane, and ethyl acetate fractions, the IC50 of the DPV method are 0.23 ± 0.00 mg/mL, 0.30 ± 0.00 mg/mL, 0.28 ± 0.00 mg/mL and 0.25 ± 0.02 mg/mL respectively while those of DPPH method are 0.39 ± 0.01 mg/mL, 0.56 ± 0.00 mg/mL, 0.51 ± 0.00 mg/mL and 0.40 ± 0.02 mg/mL respectively. The two analysis methods are strongly correlated with R2 = 0.98.

Mareya micrantha Antioxidant activity DPPH method